Antimicrobial Susceptibility of Staphylococci Isolated from Clinical Mastitis in Dairy Cows in Two Regions of Kosovo

DRITON SYLEJMANI1*, AVNI ROBAJ1, AFRIM HAMIDI1

1These authors contributed equally to this work

1University of Prishtina, Faculty of Agriculture and Veterinary, Boul. “Bill Clinton”, 10000 KOSOVO

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Abstract

This study was undertaken to evaluate antibiotic susceptibility of 26 strains of Coagulase Negative Staphylococci (CNS) and 18 strains of Staphylococcus aureus isolated from samples of dairy cows with clinical mastitis. The isolates species of staphylococci were identified by using coagulase test (using rabbit plasma) and API Staph system (bioMėrieux SA) which differentiates S. aurues from other staphylococci. Isolates were tested for antimicrobial susceptibility on Mueller Hinton agar by dick diffusion method according to the Clinical Laboratory Standards Institute (CLSI/NCCLS). The findings of this study showed that the most effective in vitro drugs for S. aureus strains isolated from cows with clinical mastitis were amoxycillin/clavulanic acid (83.3%),  tetracycline (77.8%), gentamycin (77.8%) and trimethoprim (66.7%) and for CNS strains trimethoprim (88.5%), amoxicillin-clavulanic acid (84.6%), tetracycline (77%) and gentamicin (69.2%) while the isolates of S. aureus and CNS were found to be more resistant to  penicillin (55.5% and 53.8%, respectively), streptomycin (50% and 46.1%, respectively) and ampicillin (38.9% and 46.1%, respectively).

Keywords: Antimicrobial susceptibility, staphylococci, clinical mastitis.

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