Kamel Ben Mbarek1* Mohsen Boubaker2
1Department of Horticultural Systems Engeneering, High Agronomic Institute of Chott-Mariem, IRESA-University of Sousse, Tunisia
2Department of Horticulture, High Agronomic Institute of Chott-Mariem, IRESA-University of Sousse, Tunisia
Abstract: Eight ” kabuli ” chickpea genotypes Beja1, Amdoun1, Nayer, Kasseb, Bochra, FLP96-114C, FLP88-42C and Chetoui were germinated, in two in vitro culture media, particularly, agar and filter paper Watman n°2 and under tree osmotic water pressures (OWP): -0,33; -4 and -8 bars induced by PEG8000. On filter paper, germination appeared more accelerated with a higher rate compared to the agar media. Osmotic water stress has negatively affected the seeds germination and the seedlings vegetative development parameters. Osmotic water pressure – 8 bars completely inhibited seeds germination on filter paper media. On the other hand, on agar media, it caused a feeble germination rate and a stunting of the seedlings. A broad genotypic variability of the chickpea cultivars was revealed toward the osmotic water stress. Tolerance index to osmotic water stress revealed three groups of cultivars: (1) Nayer and Kasseb are tolerant, (2) Bochra, FLIP88-42C and Chetoui are fairly tolerant and (3) Amdoun1, Beja1 and FLIP96-114C are sensitive to this abiotic stress.
Key words Chickpea; Osmotic water stress; PEG8000; Culture media.