Efficacy of freshly prepared pesta granular formulations from the multi-combination of wild and mutant strain of Lasiodiplodia pseudotheobromae  and  Pseudomonas aeruginosa AdetunjI Charles1*, and  Oloke Julius2

1Nigerian Stored Product Research Institute, Asa Dam Road, P.M.B. 1489, Ilorin, Nigeria.
2 Ladoke Akintola University of Technology, Department of Pure and Applied Biology. P.M.B 4000, Ogbomoso, Oyo State.

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Abstract:This study was carried out to determined the efficacy of freshly prepared pestal granules containing the wild and mutant strains of Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae incontrolling weeds in a potted experiment. Six multi-combination formulations of the wild and mutant strains of  Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae were combined with a semolina kaolin granular formulation which were represented as follows: BHI, BH2, BH3, BH4, BH5, and BH6. The effect of each formulated  pesta granules were supplemented with an adjuvants (glycerol, glucose,sucrose, fructose, dextrose, lactose sugar, peptone) and they were evaluated on Tridax procumbens and sorghum plant. The efficacy of the bioherbicides were in the following orders: BH4>BH2>BH6>BH3>BH1>BH5. BH4 showed the highest activity compared to other formulations by reducing  Tridax procumbens to the lowest dry weights and showing the highest percentage diseaseseverity on Tridax procumbens compared to other formulations. BH4 among other formulations also lead to  increase in the amount of  grain yield and 100-grain weight of sorghum plants compared to uninoculated plants. Therefore, the use of  pesta granules containing wild and mutant strains of Pseudomonas aeruginosa and Lasiodiplodia pseudotheobromae  exposed to U.V for 1 hour 30 minutes  against weeds using  Tridax procumbens as a study case could help in achieving a sustainable agriculture through the application of  bioherbicide produced during this study .

Keywords: bioherbicide; deleterious rhizobacteria; pesta; Lasiodiplodia pseudotheobromae; Pseudomonas aeruginosa; multi-combination.

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