Ferdi Brahushi1*, Ulrike Dörfler2, Edlira Shahinasi3, Rainer. Schroll2 And Jean-Charl Munch2
1 Department of Agro-Environment & Ecology, Agricultural University of Tirana; Tirana, Albania
2Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Soil Ecology, 85764 Neuherberg, Germany
3 Faculty of Biotechnology, Agricultural University of Tirana; Tirana, Albania
Abstract: Chlorobenzenes (CBs) are a group of substituted benzene compounds with only chlorine and hydrogen atoms on the benzene ring. They are mainly used as solvents, pesticides, fire retardants, degreasers, heat transmitters, chemical intermediates and are released in the environment due to their extensive use. This study presents a developing method for determination of chlorobenzenes in the soil, including extraction techniques, concentration, clean up and injection in GC-ECD system which was equipped with a DB-5 capillary column and an AS 2000 autosampler. The detection limit of the method was calculated based on the chlorobenzene recoveries of the presented analytical method and on the detection limits in GC of each chlorobenzes. The extraction with ASE technique presented the best results as the recoveries of MCB, 1,2,4-TCB and HCB from soil with were 100,5±2,5, 88,0±3,3 and 88,0±5,0, respectively. The detection limits of chlorobenzens in the GC-ECD varied from 0,38 pg/µl for HCB to 250,00 pg/µl for MCB. Also, the total recovery of chlorobenzenes was high for HCB (84,13 ± 4,30) and very low for MCB (9,80 ± 1,16). The values of method detection limits of chlorobenzenes in soil were from 0,14 µg/kg soil for HCB to 765,31 µg/kg soil for MCB, whereas other clorobenzenes had intermediate values. Therefore, by using this determination method, high chlorinated benzenes as HCB and PCB could be detected in very small amounts in soil samples.
Keywords: Chlorobenzenes, method, extraction, detection limit, soil.